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The Ndc80 complex bridges two Dam1 complex rings


Project Information
Title:
The Ndc80 complex bridges two Dam1 complex rings
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Project Title cannot be empty
Abstract:
Strong kinetochore-microtubule attachments are essential for faithful segregation of sister chromatids during mitosis. The Dam1 and Ndc80 complexes are the main microtubule binding components of the budding yeast kinetochore. Cooperation between these two complexes enhances kinetochore-microtubule coupling and is regulated by Aurora B kinase. We show that the Ndc80 complex can simultaneously bind and bridge across two Dam1 complex rings through a tripartite interaction, each component of which is regulated by Aurora B kinase. Mutations in any one of the Ndc80p interaction regions abrogates the Ndc80 complex’s ability to bind two Dam1 rings in vitro, and results in kinetochore biorientation and microtubule attachment defects in vivo. We also show that an extra-long Ndc80 complex, engineered to space the two Dam1 rings further apart, does not support growth. Taken together, our work suggests that each kinetochore in vivo contains two Dam1 rings and that proper ring-ring spacing is vital.
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Notes:
This page provides public access to data associated with Jae ook Kim et al. (2017)
Explore Data
[Stats/QC] [Peptides] [Proteins] [Image] [Structure]
Microtubules + Dam1 complex + Ndc80 complex cross-linked with DSS. Raw data for Figure 1 (313)
Linker: dss
Search Programs:
kojak 1.4.2
percolator 2.08
Upload: 2016-01-27 21:55:39
FASTA file: EColiContamDam1MindCOMA-Spc110-Ndc80-SspH1-NewCowTubulin.fasta
Cutoffs Applied On Import:
Peptide Cutoff: q-value 0.05
PSM Cutoff: q-value 0.05
Raw MS data files:
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Web Link URL is invalid
Q_2014_0214_RJ_43_29s.mzML
Q_2014_0214_RJ_44_29.mzML
Q_2014_0214_RJ_45_29.mzML
Q_2014_0214_RJ_46_29.mzML
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Additional files:
Kojak-1.4.2-file1.conf
Kojak-1.4.2-file2.conf
Kojak-1.4.2-file3.conf
Kojak-1.4.2-file4.conf
[Stats/QC] [Peptides] [Proteins] [Image] [Structure]
Microtubules + Dam1 complex + Ndc80 complex cross-linked with EDC. Raw data for Figure 1 (312)
Linker: edc
Search Programs:
kojak 1.4.2
percolator 2.08
Upload: 2016-01-27 11:16:26
FASTA file: EColiContamDam1MindCOMA-Ndc80-SspH1-NewCowTubulin.fasta
Cutoffs Applied On Import:
Peptide Cutoff: q-value 0.05
PSM Cutoff: q-value 0.05
Raw MS data files:
X
Web Link URL is invalid
QEP2_2015_0717_RJ_61_rjaz140_xlink5.mzML
QEP2_2015_0717_RJ_62_rjaz140_xlink9.mzML
QEP2_2015_0717_RJ_87_rjaz140_comet.mzML
QEP2_2015_0717_RJ_88_rjaz140_xlink7.mzML
{{linkLabel}}
Additional files:
Kojak-1.4.2-file1.conf
Kojak-1.4.2-file2.conf
Kojak-1.4.2-file3.conf
Kojak-1.4.2-file4.conf
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