Project Information
Title:
A Stu2-mediated tension-sensing pathway promotes chromosome biorientation in vivo
Abstract:
The accurate segregation of chromosomes to daughter cells is a critical aspect of cell division. It requires the kinetochores on duplicated chromosomes to biorient, attaching to microtubules from opposite poles of the cell. Bioriented attachments are ensured by the cell’s error correction machinery, which functions by selectively stabilizing tension-bearing attachments. The conserved Aurora B kinase’s role in error correction is to destabilize low tension-bearing attachments. We recently discovered that in vitro, kinetochores display an additional intrinsic tension-sensing pathway that utilizes Stu2; however, this pathway’s contribution to error correction in cells was unknown. Here, we identify a Stu2 mutant that abolishes its function at kinetochores and found this results in error correction defects in vivo. We also discovered that this intrinsic tension-sensing pathway functions in concert with the Aurora B-mediated pathway. Together, our work indicates that cells employ at least two pathways to ensure biorientation and the accuracy of chromosome segregation.
Notes:
This page provides public access to data associated with Miller et al. (2019)
