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The molecular architecture of the Dam1 kinetochore complex is defined by cross-l...


Project Information
Title:
The molecular architecture of the Dam1 kinetochore complex is defined by cross-linking based structural modelling.
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Project Title cannot be empty
Abstract:
Accurate segregation of chromosomes during cell division is essential. The Dam1 complex binds kinetochores to microtubules and its oligomerization is required to form strong attachments. It is a key target of Aurora B kinase, which destabilizes erroneous attachments allowing subsequent correction. Understanding the roles and regulation of the Dam1 complex requires structural information. Here we apply cross-linking/mass spectrometry and structural modelling to determine the molecular architecture of the Dam1 complex. We find microtubule attachment is accompanied by substantial conformational changes, with direct binding mediated by the carboxy termini of Dam1p and Duo1p. Aurora B phosphorylation of Dam1p C terminus weakens direct interaction with the microtubule. Furthermore, the Dam1p amino terminus forms an interaction interface between Dam1 complexes, which is also disrupted by phosphorylation. Our results demonstrate that Aurora B inhibits both direct interaction with the microtubule and oligomerization of the Dam1 complex to drive error correction during mitosis.
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Notes:
This page provides public access to data associated with Zelter et al. (2015)
Explore Data
[Stats/QC] [Peptides] [Proteins] [Image] [Structure]
γ-TuSC cross-linked with DSS. Raw Data for Supplemental Table 2 (185)
Linker: dss
Search Programs:
kojak 1.0
percolator Version Unknown
Upload: 2015-06-10 22:32:53
FASTA file: 2012-10-yates-His-Tub4-110-plusBleed-PlusRev.fasta
Cutoffs Applied On Import:
Peptide Cutoff: q-value 0.05
PSM Cutoff: q-value 0.05
Raw MS data files:
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Web Link URL is invalid
Q_2013_1010_RJ_07.raw
Q_2013_1010_RJ_08.raw
Q_2013_1010_RJ_09.raw
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Additional files:
KojakTub4-1.conf
KojakTub4-2.conf
KojakTub4-3.conf
[Stats/QC] [Peptides] [Proteins] [Image] [Structure]
BARD1/BRAC1 cross-linked with DSS. Raw Data for Supplemental Table 3 (187)
Linker: dss
Search Programs:
kojak 1.0
percolator Version Unknown
Upload: 2015-06-10 23:40:52
FASTA file: 2014-06-fly-ecoli-contam-plus.fasta
Cutoffs Applied On Import:
Peptide Cutoff: q-value 0.05
PSM Cutoff: q-value 0.05
Raw MS data files:
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Web Link URL is invalid
QEP_2014_0613_RJ_55_63xlink02.raw
QEP_2014_0613_RJ_56_63xlink05.raw
QEP_2014_0613_RJ_57_63xlink07.raw
QEP_2014_0613_RJ_58_63xlink02b.raw
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Additional files:
KojakBARD-1.conf
KojakBARD-2.conf
KojakBARD-3.conf
KojakBARD-4.conf
[Stats/QC] [Peptides] [Proteins] [Image] [Structure]
SCFFBXL3 cross-linked with DSS. Raw Data for Supplemental Table 4 (186)
Linker: dss
Search Programs:
kojak 1.0
percolator Version Unknown
Upload: 2015-06-10 23:04:00
FASTA file: RJAZ10-comet-plus.fasta
Cutoffs Applied On Import:
Peptide Cutoff: q-value 0.05
PSM Cutoff: q-value 0.05
Raw MS data files:
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Web Link URL is invalid
QEP_2014_0416_RJ_15_24_xlink01.raw
QEP_2014_0416_RJ_16_24_xlink02.raw
QEP_2014_0416_RJ_17_24_xlink03.raw
QEP_2014_0416_RJ_18_24_xlink04.raw
QEP_2014_0416_RJ_30_24_xlink05_1.raw
QEP_2014_0416_RJ_31_24_xlink06_1.raw
QEP_2014_0416_RJ_32_24_xlink07_1.raw
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Additional files:
KojakCrypto-1.conf
KojakCrypto-2.conf
KojakCrypto-3.conf
KojakCrypto-4.conf
KojakCrypto-5.conf
KojakCrypto-6.conf
KojakCrypto-7.conf
[Stats/QC] [Peptides] [Proteins] [Image] [Structure]
Free Dam1 complex cross-linked with DSS. Raw Data for Supplemental Table 6 (184)
Linker: dss
Search Programs:
kojak 1.0
percolator Version Unknown
Upload: 2015-06-10 22:09:46
FASTA file: RJAZ08-alex-comet-plus.fasta
Cutoffs Applied On Import:
Peptide Cutoff: q-value 0.05
PSM Cutoff: q-value 0.05
Raw MS data files:
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Web Link URL is invalid
Q_2013_1010_RJ_36.raw
Q_2013_1010_RJ_37.raw
Q_2013_1010_RJ_38.raw
Q_2013_1010_RJ_39.raw
Q_2013_1023_RJ_36_zelter20sequest.raw
Q_2013_1023_RJ_37_zelter20.raw
Q_2013_1023_RJ_38_zelter20.raw
Q_2013_1023_RJ_39_zelter20.raw
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Additional files:
KojakDam1free-1.conf
KojakDam1free-2.conf
KojakDam1free-3.conf
KojakDam1free-4.conf
KojakDam1free-5.conf
KojakDam1free-6.conf
KojakDam1free-7.conf
KojakDam1free-8.conf
[Stats/QC] [Peptides] [Proteins] [Image] [Structure]
Dam1 + microtubules cross-linked with DSS. Raw Data for Supplemental Table 6 (188)
Linker: dss
Search Programs:
kojak 1.0
percolator Version Unknown
Upload: 2015-06-11 10:40:29
FASTA file: RJAZ28-filterFasta-Plusrev.fasta
Cutoffs Applied On Import:
Peptide Cutoff: q-value 0.05
PSM Cutoff: q-value 0.05
Raw MS data files:
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Web Link URL is invalid
QEP_2014_0416_RJ_80_28_xlink01.raw
QEP_2014_0416_RJ_81_28_xlink02.raw
QEP_2014_0416_RJ_82_28_xlink04.raw
QEP_2014_0416_RJ_83_28_xlink07.raw
QEP_2014_0416_RJ_84_28_xlink02_60m.raw
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Additional files:
KojakDam1MT-1.conf
KojakDam1MT-2.conf
KojakDam1MT-3.conf
KojakDam1MT-4.conf
KojakDam1MT-5.conf
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Managed by Michael Riffle (mriffle@uw.edu)